Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5105 | 5' | -49.9 | NC_001798.1 | + | 137281 | 0.66 | 0.995263 |
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Target: 5'- cGGuCCCCGGCgauggcCUGUUUgagGAUGGc- -3' miRNA: 3'- -CC-GGGGCCGa-----GACAAGauaCUAUUuc -5' |
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| 5105 | 5' | -49.9 | NC_001798.1 | + | 87519 | 0.67 | 0.994479 |
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Target: 5'- uGCCCCGGCUaccgGUUCgccgcccacgUGUGGcAGAGc -3' miRNA: 3'- cCGGGGCCGAga--CAAG----------AUACUaUUUC- -5' |
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| 5105 | 5' | -49.9 | NC_001798.1 | + | 5310 | 0.69 | 0.979537 |
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Target: 5'- cGCCCCGGCUCgccgggGUUCcgcccccaGGUGGAa -3' miRNA: 3'- cCGGGGCCGAGa-----CAAGaua-----CUAUUUc -5' |
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| 5105 | 5' | -49.9 | NC_001798.1 | + | 68654 | 0.69 | 0.968657 |
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Target: 5'- uGGUCCCGGCaCUGUcg-GUaGGUGAAGa -3' miRNA: 3'- -CCGGGGCCGaGACAagaUA-CUAUUUC- -5' |
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| 5105 | 5' | -49.9 | NC_001798.1 | + | 119428 | 0.71 | 0.935759 |
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Target: 5'- uGGCCCCGGCg--GUUCg--GGUGu-- -3' miRNA: 3'- -CCGGGGCCGagaCAAGauaCUAUuuc -5' |
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| 5105 | 5' | -49.9 | NC_001798.1 | + | 108109 | 0.79 | 0.566079 |
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Target: 5'- gGGCCCCGG-UCUGUgggugGUGAUGGGGg -3' miRNA: 3'- -CCGGGGCCgAGACAaga--UACUAUUUC- -5' |
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| 5105 | 5' | -49.9 | NC_001798.1 | + | 148807 | 1.12 | 0.006915 |
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Target: 5'- gGGCCCCGGCUCUGUUCUAUGAUAAAGa -3' miRNA: 3'- -CCGGGGCCGAGACAAGAUACUAUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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