Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5110 | 3' | -58.5 | NC_001798.1 | + | 90395 | 0.68 | 0.717501 |
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Target: 5'- gGGGGGCGCGguugggCCggcgcguUCCCGCGGCcgggcuugaGGCGGu -3' miRNA: 3'- -CUCUUGUGUa-----GG-------AGGGCGCCG---------CCGUC- -5' |
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| 5110 | 3' | -58.5 | NC_001798.1 | + | 149717 | 0.68 | 0.71848 |
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Target: 5'- --uGGCACAcuUCCUcggccCCCGCGGCGcaGCAGc -3' miRNA: 3'- cucUUGUGU--AGGA-----GGGCGCCGC--CGUC- -5' |
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| 5110 | 3' | -58.5 | NC_001798.1 | + | 9151 | 0.68 | 0.71848 |
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Target: 5'- -cGGGCGCGgcgCCgCCCGCgccgGGgGGCAGg -3' miRNA: 3'- cuCUUGUGUa--GGaGGGCG----CCgCCGUC- -5' |
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| 5110 | 3' | -58.5 | NC_001798.1 | + | 12606 | 0.68 | 0.71848 |
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Target: 5'- uGGGGGC-CAUCUgagCGCGGCGGCGu -3' miRNA: 3'- -CUCUUGuGUAGGaggGCGCCGCCGUc -5' |
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| 5110 | 3' | -58.5 | NC_001798.1 | + | 52184 | 0.67 | 0.727258 |
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Target: 5'- cGGGGCGCGUCCUggacgugCUgGCGGUccuGGCGGa -3' miRNA: 3'- cUCUUGUGUAGGA-------GGgCGCCG---CCGUC- -5' |
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| 5110 | 3' | -58.5 | NC_001798.1 | + | 39421 | 0.67 | 0.728229 |
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Target: 5'- cGGGGCGCggCCgUCCGCGuGCGGgGGg -3' miRNA: 3'- cUCUUGUGuaGGaGGGCGC-CGCCgUC- -5' |
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| 5110 | 3' | -58.5 | NC_001798.1 | + | 105392 | 0.67 | 0.728229 |
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Target: 5'- gGAGGGCgGCGUUCUCcagggccgCCGCGGCcGCGGu -3' miRNA: 3'- -CUCUUG-UGUAGGAG--------GGCGCCGcCGUC- -5' |
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| 5110 | 3' | -58.5 | NC_001798.1 | + | 150558 | 0.67 | 0.737898 |
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Target: 5'- gGAGAGCGgc-CCggggCCCGCGG-GGCGGc -3' miRNA: 3'- -CUCUUGUguaGGa---GGGCGCCgCCGUC- -5' |
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| 5110 | 3' | -58.5 | NC_001798.1 | + | 48287 | 0.66 | 0.819839 |
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Target: 5'- cGAGGGCAagaaCCUCCUGCaGCGcGCGa -3' miRNA: 3'- -CUCUUGUgua-GGAGGGCGcCGC-CGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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