Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5111 | 5' | -55.2 | NC_001798.1 | + | 137944 | 0.67 | 0.902542 |
Target: 5'- -gCC-GCCCGGUcc--GCCGCGCGCu -3' miRNA: 3'- caGGaCGGGCUAcuauUGGUGCGCGu -5' |
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5111 | 5' | -55.2 | NC_001798.1 | + | 140835 | 0.71 | 0.704649 |
Target: 5'- cGUCCUcaacaGCCaGGUcgcGGUGACCGCGCGCc -3' miRNA: 3'- -CAGGA-----CGGgCUA---CUAUUGGUGCGCGu -5' |
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5111 | 5' | -55.2 | NC_001798.1 | + | 144095 | 1.08 | 0.003822 |
Target: 5'- gGUCCUGCCCGAUGAUAACCACGCGCAc -3' miRNA: 3'- -CAGGACGGGCUACUAUUGGUGCGCGU- -5' |
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5111 | 5' | -55.2 | NC_001798.1 | + | 153027 | 0.66 | 0.914821 |
Target: 5'- aGUCCggGCCCGcgc--GGCgGCGCGCGg -3' miRNA: 3'- -CAGGa-CGGGCuacuaUUGgUGCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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