miRNA: 3'- -UCCUCUUaGAGCGCCUUGUuguaGCa -5'
miRNA: 3'- -UCCUCUUaGAGcGCCU---UGUUGUAGCa -5'
miRNA: 3'- -UCCUCuUAGAGCGCCuugUugUAGCA- -5'
miRNA: 3'- -UCCUCUUAGAGCGCCUUGUUGUAGCA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5122 | 5' | -50 | NC_001798.1 | + | 139374 | 0.7 | 0.967465 |
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Target: 5'- gGGGAGGG-CUCGCGGGACGuc--CGg -3' miRNA: 3'- -UCCUCUUaGAGCGCCUUGUuguaGCa -5' |
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| 5122 | 5' | -50 | NC_001798.1 | + | 16721 | 0.71 | 0.928629 |
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Target: 5'- cGGGGGAAcCUCcCGGGaaaACAACAUCGc -3' miRNA: 3'- -UCCUCUUaGAGcGCCU---UGUUGUAGCa -5' |
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| 5122 | 5' | -50 | NC_001798.1 | + | 88841 | 0.73 | 0.884176 |
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Target: 5'- gAGGAGcAUCUCGCGGccgAugGUCGUc -3' miRNA: 3'- -UCCUCuUAGAGCGCCuugUugUAGCA- -5' |
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| 5122 | 5' | -50 | NC_001798.1 | + | 139229 | 1.08 | 0.013513 |
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Target: 5'- gAGGAGAAUCUCGCGGAACAACAUCGUc -3' miRNA: 3'- -UCCUCUUAGAGCGCCUUGUUGUAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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