miRNA: 3'- caGGGGCguCGCGAa------UUUCauUAGGA- -5'
miRNA: 3'- -CAgGGGCG---UCGCGAAUUUCAUU---AGGA- -5'
miRNA: 3'- cagGGGCGUCGCGa-----AUUUCAUUAGGA- -5'
miRNA: 3'- -CAGGGGCGUCGCGAAUUUCAUUAGGA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5126 | 5' | -51.3 | NC_001798.1 | + | 91108 | 0.74 | 0.743464 |
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Target: 5'- -aCCCCGguGCGCUgcuccgcGGGGgcGUCCUc -3' miRNA: 3'- caGGGGCguCGCGAa------UUUCauUAGGA- -5' |
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| 5126 | 5' | -51.3 | NC_001798.1 | + | 66109 | 0.74 | 0.722987 |
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Target: 5'- aGUgCCCGCcgaAGCGCUUGAGGUAAaacUCUUc -3' miRNA: 3'- -CAgGGGCG---UCGCGAAUUUCAUU---AGGA- -5' |
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| 5126 | 5' | -51.3 | NC_001798.1 | + | 18135 | 0.74 | 0.712615 |
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Target: 5'- --aCCCGCGGCGUccacgaUGGGGUAGUCCUc -3' miRNA: 3'- cagGGGCGUCGCGa-----AUUUCAUUAGGA- -5' |
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| 5126 | 5' | -51.3 | NC_001798.1 | + | 137066 | 1.07 | 0.009152 |
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Target: 5'- gGUCCCCGCAGCGCUUAAAGUAAUCCUc -3' miRNA: 3'- -CAGGGGCGUCGCGAAUUUCAUUAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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