Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5134 | 3' | -65.5 | NC_001798.1 | + | 147036 | 0.75 | 0.12138 |
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Target: 5'- -uUGGCCgCCGAGGUGcgccccggccggaGGGGCCC-CCGc -3' miRNA: 3'- cuACCGG-GGCUCCAC-------------CCCCGGGuGGU- -5' |
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| 5134 | 3' | -65.5 | NC_001798.1 | + | 34810 | 0.76 | 0.110506 |
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Target: 5'- --gGGCCCCGGGGcccccgcgcuccgccGGGGGCCCggGCCGg -3' miRNA: 3'- cuaCCGGGGCUCCa--------------CCCCCGGG--UGGU- -5' |
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| 5134 | 3' | -65.5 | NC_001798.1 | + | 131371 | 0.76 | 0.109959 |
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Target: 5'- --cGGCCUCGGGGgauccccUGGGGGCCCGgCGu -3' miRNA: 3'- cuaCCGGGGCUCC-------ACCCCCGGGUgGU- -5' |
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| 5134 | 3' | -65.5 | NC_001798.1 | + | 147498 | 0.76 | 0.102317 |
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Target: 5'- --aGGCUCCGGGGgggGGGGGCgCCugCGu -3' miRNA: 3'- cuaCCGGGGCUCCa--CCCCCG-GGugGU- -5' |
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| 5134 | 3' | -65.5 | NC_001798.1 | + | 31538 | 0.77 | 0.090304 |
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Target: 5'- --gGGCCCCGGGGgGGGaGGUCUGCCAc -3' miRNA: 3'- cuaCCGGGGCUCCaCCC-CCGGGUGGU- -5' |
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| 5134 | 3' | -65.5 | NC_001798.1 | + | 3885 | 0.78 | 0.075719 |
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Target: 5'- --cGcGCCCCGGGGgcgGGGGGCCgGCCc -3' miRNA: 3'- cuaC-CGGGGCUCCa--CCCCCGGgUGGu -5' |
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| 5134 | 3' | -65.5 | NC_001798.1 | + | 34975 | 0.78 | 0.073829 |
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Target: 5'- --cGGCCgCCGAGGUgcGGGGGCCCcuccgGCCGg -3' miRNA: 3'- cuaCCGG-GGCUCCA--CCCCCGGG-----UGGU- -5' |
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| 5134 | 3' | -65.5 | NC_001798.1 | + | 130538 | 1.07 | 0.000506 |
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Target: 5'- cGAUGGCCCCGAGGUGGGGGCCCACCAa -3' miRNA: 3'- -CUACCGGGGCUCCACCCCCGGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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