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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5161 | 3' | -52.6 | NC_001798.1 | + | 138501 | 0.67 | 0.96751 |
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Target: 5'- --cCGGAGGGCGUG-GCAcgcgacgaucUCGGGGc -3' miRNA: 3'- cuuGCUUCCCGCACaCGUca--------AGCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 100978 | 0.67 | 0.970551 |
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Target: 5'- --cCGAccccgGGGGCGUGgGCGGggaGGGGg -3' miRNA: 3'- cuuGCU-----UCCCGCACaCGUCaagCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 35386 | 0.67 | 0.971709 |
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Target: 5'- aAugGAAGGGCGUGgggccggccgccggaUGCccg-CGGGGg -3' miRNA: 3'- cUugCUUCCCGCAC---------------ACGucaaGCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 146040 | 0.66 | 0.976012 |
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Target: 5'- --gUGggGGGCGUgGUGguGaaCGAuGGg -3' miRNA: 3'- cuuGCuuCCCGCA-CACguCaaGCU-CC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 2538 | 0.66 | 0.978446 |
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Target: 5'- gGGGCGggGGGCGcGgcccccGCGGga-GGGGc -3' miRNA: 3'- -CUUGCuuCCCGCaCa-----CGUCaagCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 31146 | 0.66 | 0.978446 |
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Target: 5'- cGGCGcGGGGGCGgcgGUGCGGgggCGAc- -3' miRNA: 3'- cUUGC-UUCCCGCa--CACGUCaa-GCUcc -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 34684 | 0.66 | 0.978446 |
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Target: 5'- aGAGgGAAgGGGCGcG-GCGGgaCGGGGg -3' miRNA: 3'- -CUUgCUU-CCCGCaCaCGUCaaGCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 51012 | 0.66 | 0.978446 |
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Target: 5'- aGGACGAgcccgAGGGCG-GUGgAGU-CGGGu -3' miRNA: 3'- -CUUGCU-----UCCCGCaCACgUCAaGCUCc -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 12927 | 0.66 | 0.980037 |
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Target: 5'- cGGCGAuuuuuaagcuguauAGGGCGacgGUGCgcuGGUuuUCGGGGa -3' miRNA: 3'- cUUGCU--------------UCCCGCa--CACG---UCA--AGCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 136872 | 0.66 | 0.984654 |
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Target: 5'- cAGCG-AGGGCGUG-GCGuGgcucCGGGGa -3' miRNA: 3'- cUUGCuUCCCGCACaCGU-Caa--GCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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