miRNA: 3'- -CCA---CCGCGGcCGUACauaauaUCGGC- -5'
miRNA: 3'- ccaCCGCGGCC--------------GUACAUa---AuaUCGGC- -5'
miRNA: 3'- -CCACCG-------CGGCCGUACauaauauCGGC- -5'
miRNA: 3'- ccaCCG-CGGCCGUACAuaaUAUCGGc -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5162 | 3' | -52.8 | NC_001798.1 | + | 28663 | 0.66 | 0.976197 |
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Target: 5'- cGGUuccGGCGCC-GCGUGgcggc-GGCCGa -3' miRNA: 3'- -CCA---CCGCGGcCGUACauaauaUCGGC- -5' |
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| 5162 | 3' | -52.8 | NC_001798.1 | + | 29599 | 0.66 | 0.980215 |
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Target: 5'- --aGGCGCCGGguauaaggcagcccCGUGUGacggUcgGGCCGc -3' miRNA: 3'- ccaCCGCGGCC--------------GUACAUa---AuaUCGGC- -5' |
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| 5162 | 3' | -52.8 | NC_001798.1 | + | 36042 | 0.66 | 0.980652 |
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Target: 5'- gGGUGGCggcggugGUCGGCGUGcu-----GCCGg -3' miRNA: 3'- -CCACCG-------CGGCCGUACauaauauCGGC- -5' |
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| 5162 | 3' | -52.8 | NC_001798.1 | + | 68783 | 0.66 | 0.980868 |
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Target: 5'- --gGGUgGCCGGUGUGU---GUGGCCu -3' miRNA: 3'- ccaCCG-CGGCCGUACAuaaUAUCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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