Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5175 | 5' | -54.6 | NC_001798.1 | + | 122358 | 0.66 | 0.959475 |
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Target: 5'- cGGUGGcGUGggGCgGGCGACGc----- -3' miRNA: 3'- -CCACC-CACagCGgCCGCUGUuuacuc -5' |
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| 5175 | 5' | -54.6 | NC_001798.1 | + | 130350 | 0.75 | 0.539755 |
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Target: 5'- aGUGuGGUGUCGCCGGUGAUAc----- -3' miRNA: 3'- cCAC-CCACAGCGGCCGCUGUuuacuc -5' |
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| 5175 | 5' | -54.6 | NC_001798.1 | + | 144825 | 0.67 | 0.928542 |
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Target: 5'- -cUGGGUGUCgGCCGGaaACAcagAGUGAc -3' miRNA: 3'- ccACCCACAG-CGGCCgcUGU---UUACUc -5' |
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| 5175 | 5' | -54.6 | NC_001798.1 | + | 148410 | 0.71 | 0.770373 |
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Target: 5'- cGGgcgGGGUGggCGCCGG-GGCGggGGUGGGc -3' miRNA: 3'- -CCa--CCCACa-GCGGCCgCUGU--UUACUC- -5' |
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| 5175 | 5' | -54.6 | NC_001798.1 | + | 149247 | 0.67 | 0.911734 |
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Target: 5'- cGG-GGGcGUCGCCGGcCGGCGc--GGGc -3' miRNA: 3'- -CCaCCCaCAGCGGCC-GCUGUuuaCUC- -5' |
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| 5175 | 5' | -54.6 | NC_001798.1 | + | 153007 | 0.66 | 0.951799 |
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Target: 5'- --cGGGgcgGUCGCCGG-GGCGGAguccGGGc -3' miRNA: 3'- ccaCCCa--CAGCGGCCgCUGUUUa---CUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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