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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5194 | 3' | -65 | NC_001798.1 | + | 31667 | 0.73 | 0.175099 |
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Target: 5'- -gGCCGGGCCCgCGccgcccgCCgUGCCGGUg -3' miRNA: 3'- aaCGGCCCGGGgGCaa-----GG-ACGGCCAg -5' |
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| 5194 | 3' | -65 | NC_001798.1 | + | 27919 | 0.66 | 0.45229 |
|
Target: 5'- -cGCCgGGGUCCCCG--CC-GCCGGg- -3' miRNA: 3'- aaCGG-CCCGGGGGCaaGGaCGGCCag -5' |
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| 5194 | 3' | -65 | NC_001798.1 | + | 27719 | 0.67 | 0.409778 |
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Target: 5'- -gGCgGGaGUCCCCG-UCCUGCCGc-- -3' miRNA: 3'- aaCGgCC-CGGGGGCaAGGACGGCcag -5' |
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| 5194 | 3' | -65 | NC_001798.1 | + | 24859 | 0.68 | 0.347194 |
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Target: 5'- -cGCCGuGGCCgCCGUgcgcgCCgugaGCCuGGUCg -3' miRNA: 3'- aaCGGC-CCGGgGGCAa----GGa---CGG-CCAG- -5' |
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| 5194 | 3' | -65 | NC_001798.1 | + | 23714 | 0.68 | 0.369844 |
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Target: 5'- -gGCCGGGCggCCCCGgcgggUCgaGCUGGa- -3' miRNA: 3'- aaCGGCCCG--GGGGCa----AGgaCGGCCag -5' |
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| 5194 | 3' | -65 | NC_001798.1 | + | 23664 | 0.66 | 0.45229 |
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Target: 5'- -cGCCGGGCCCgCGcggCggugGCCGGcCg -3' miRNA: 3'- aaCGGCCCGGGgGCaagGa---CGGCCaG- -5' |
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| 5194 | 3' | -65 | NC_001798.1 | + | 20017 | 0.66 | 0.48797 |
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Target: 5'- -gGCCggggaGGGCCCCCccUCCgUGUCGGa- -3' miRNA: 3'- aaCGG-----CCCGGGGGcaAGG-ACGGCCag -5' |
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| 5194 | 3' | -65 | NC_001798.1 | + | 5547 | 0.66 | 0.469957 |
|
Target: 5'- aUGCCGGccccgcGCUCCCGUUggccCCcGCCGGcCc -3' miRNA: 3'- aACGGCC------CGGGGGCAA----GGaCGGCCaG- -5' |
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| 5194 | 3' | -65 | NC_001798.1 | + | 3026 | 0.67 | 0.426487 |
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Target: 5'- -gGCCGGGCuccggccagCCCCGgcacggCC-GCCaGGUCg -3' miRNA: 3'- aaCGGCCCG---------GGGGCaa----GGaCGG-CCAG- -5' |
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| 5194 | 3' | -65 | NC_001798.1 | + | 2470 | 0.67 | 0.401578 |
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Target: 5'- -gGUCgGGGCCCUCGgcgggCCgGCgGGUCa -3' miRNA: 3'- aaCGG-CCCGGGGGCaa---GGaCGgCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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