Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5198 | 3' | -60.4 | NC_001798.1 | + | 7966 | 0.67 | 0.699298 |
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Target: 5'- gCAUGCGCCUucugGUGCUuuuggcgcacgCGUCGAcguguuccuacGCGGGGu -3' miRNA: 3'- -GUACGCGGG----CAUGG-----------GCAGCU-----------CGCCCU- -5' |
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| 5198 | 3' | -60.4 | NC_001798.1 | + | 24870 | 0.67 | 0.699298 |
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Target: 5'- cCGUGCGCgCCGUgaGCCUGgUCGc-CGGGGc -3' miRNA: 3'- -GUACGCG-GGCA--UGGGC-AGCucGCCCU- -5' |
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| 5198 | 3' | -60.4 | NC_001798.1 | + | 27110 | 0.67 | 0.708957 |
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Target: 5'- gGUG-GCCCGaGCCCccccgcagGAGCGGGAg -3' miRNA: 3'- gUACgCGGGCaUGGGcag-----CUCGCCCU- -5' |
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| 5198 | 3' | -60.4 | NC_001798.1 | + | 25865 | 0.67 | 0.708957 |
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Target: 5'- cCGUGCGCgCCGcggacugGCCCGcCGA-CGGGc -3' miRNA: 3'- -GUACGCG-GGCa------UGGGCaGCUcGCCCu -5' |
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| 5198 | 3' | -60.4 | NC_001798.1 | + | 152873 | 0.66 | 0.765351 |
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Target: 5'- --gGCgGCCCGUuaaaagcugcuAauuaCCG-CGAGCGGGAa -3' miRNA: 3'- guaCG-CGGGCA-----------Ug---GGCaGCUCGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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