Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5201 | 5' | -52.5 | NC_001798.1 | + | 104763 | 0.7 | 0.864848 |
Target: 5'- cCGcGGcCAGACGCGccagguaGCGCGGAGGc- -3' miRNA: 3'- aGCaCCaGUCUGCGU-------UGCGUCUCUuu -5' |
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5201 | 5' | -52.5 | NC_001798.1 | + | 45340 | 0.71 | 0.841379 |
Target: 5'- aCGUGGUCAuggaggacGugGCGAuCGCGGAGc-- -3' miRNA: 3'- aGCACCAGU--------CugCGUU-GCGUCUCuuu -5' |
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5201 | 5' | -52.5 | NC_001798.1 | + | 87567 | 1.06 | 0.008234 |
Target: 5'- cUCGUGGUCAGACGCAACGCAGAGAAAc -3' miRNA: 3'- -AGCACCAGUCUGCGUUGCGUCUCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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