Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5202 | 3' | -54.3 | NC_001798.1 | + | 83654 | 0.66 | 0.950483 |
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Target: 5'- gGAUGUGCGgC-UCGCGGGcGucUGGGAu -3' miRNA: 3'- gCUACACGCgGuAGUGCCU-Cu-ACCUU- -5' |
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| 5202 | 3' | -54.3 | NC_001798.1 | + | 28581 | 0.66 | 0.946142 |
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Target: 5'- ---gGUGCGCCAUCugGuGGccUGGGAg -3' miRNA: 3'- gcuaCACGCGGUAGugC-CUcuACCUU- -5' |
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| 5202 | 3' | -54.3 | NC_001798.1 | + | 78052 | 0.66 | 0.936729 |
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Target: 5'- gCGcgGcUGCuGCgCAUCGCGGAGGggcuccUGGAAa -3' miRNA: 3'- -GCuaC-ACG-CG-GUAGUGCCUCU------ACCUU- -5' |
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| 5202 | 3' | -54.3 | NC_001798.1 | + | 24562 | 0.66 | 0.931652 |
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Target: 5'- uGGUGgcgGCgGCCGgcgCGCGGAGgcGGGc -3' miRNA: 3'- gCUACa--CG-CGGUa--GUGCCUCuaCCUu -5' |
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| 5202 | 3' | -54.3 | NC_001798.1 | + | 100717 | 0.67 | 0.895996 |
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Target: 5'- gCGGggcUGUGCGCguUCuucgaGGGGAUGGGGg -3' miRNA: 3'- -GCU---ACACGCGguAGug---CCUCUACCUU- -5' |
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| 5202 | 3' | -54.3 | NC_001798.1 | + | 39458 | 0.7 | 0.772164 |
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Target: 5'- -----aGCGCCAUCAgCGGAGggGGGg -3' miRNA: 3'- gcuacaCGCGGUAGU-GCCUCuaCCUu -5' |
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| 5202 | 3' | -54.3 | NC_001798.1 | + | 148416 | 0.76 | 0.440212 |
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Target: 5'- gGGUGgGCGCCGggGCGGGGGUGGGc -3' miRNA: 3'- gCUACaCGCGGUagUGCCUCUACCUu -5' |
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| 5202 | 3' | -54.3 | NC_001798.1 | + | 86526 | 1.07 | 0.005373 |
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Target: 5'- cCGAUGUGCGCCAUCACGGAGAUGGAAg -3' miRNA: 3'- -GCUACACGCGGUAGUGCCUCUACCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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