Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5204 | 3' | -53.9 | NC_001798.1 | + | 102280 | 0.67 | 0.909959 |
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Target: 5'- gGCuCGUCAcGGccGCAGUcguACUCCGGCUGc -3' miRNA: 3'- -CG-GCGGUuCCa-CGUCA---UGAGGUUGAU- -5' |
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| 5204 | 3' | -53.9 | NC_001798.1 | + | 54703 | 0.67 | 0.902992 |
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Target: 5'- gGCCGCCGGGGUcuacgacGCGGUGCggaCCu---- -3' miRNA: 3'- -CGGCGGUUCCA-------CGUCAUGa--GGuugau -5' |
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| 5204 | 3' | -53.9 | NC_001798.1 | + | 90388 | 0.68 | 0.890259 |
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Target: 5'- cGCCGUCGGGGggcGCGGUugggCCGGCg- -3' miRNA: 3'- -CGGCGGUUCCa--CGUCAuga-GGUUGau -5' |
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| 5204 | 3' | -53.9 | NC_001798.1 | + | 74288 | 0.68 | 0.875922 |
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Target: 5'- aUCGCCAAGGUGCGGgcggGCgaCGugUAc -3' miRNA: 3'- cGGCGGUUCCACGUCa---UGagGUugAU- -5' |
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| 5204 | 3' | -53.9 | NC_001798.1 | + | 67560 | 0.68 | 0.875922 |
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Target: 5'- cGCCGCCAGGGgggGCGac-CUCgGGCUc -3' miRNA: 3'- -CGGCGGUUCCa--CGUcauGAGgUUGAu -5' |
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| 5204 | 3' | -53.9 | NC_001798.1 | + | 85734 | 1.09 | 0.003758 |
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Target: 5'- cGCCGCCAAGGUGCAGUACUCCAACUAu -3' miRNA: 3'- -CGGCGGUUCCACGUCAUGAGGUUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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