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miRNA: 3'- -GCGUC----AGGCGuUGG-GCCCGGGa---GUAG- -5'
miRNA: 3'- gCGUCAGGCguUGGGCCCGGGaGUag -5'
miRNA: 3'- gCG-UCAGGCGU--UGGG---CCCGGGAGUAG- -5'
miRNA: 3'- -GCGUCAGGCGUUGGGCCCGGGAGUAG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 5204 | 5' | -60.5 | NC_001798.1 | + | 28803 | 0.75 | 0.236826 |
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Target: 5'- gCGCGGguccUCCGCcGCCgCGGGCCCgggcCGUCg -3' miRNA: 3'- -GCGUC----AGGCGuUGG-GCCCGGGa---GUAG- -5' |
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| 5204 | 5' | -60.5 | NC_001798.1 | + | 147207 | 0.76 | 0.215394 |
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Target: 5'- gGCGGUCCG--GCCCGGGCCCcCGg- -3' miRNA: 3'- gCGUCAGGCguUGGGCCCGGGaGUag -5' |
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| 5204 | 5' | -60.5 | NC_001798.1 | + | 86650 | 0.78 | 0.164885 |
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Target: 5'- gGCgGGUCUGUAuaGCCCugcGGGCCCUCAUCu -3' miRNA: 3'- gCG-UCAGGCGU--UGGG---CCCGGGAGUAG- -5' |
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| 5204 | 5' | -60.5 | NC_001798.1 | + | 85771 | 1.09 | 0.001031 |
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Target: 5'- gCGCAGUCCGCAACCCGGGCCCUCAUCg -3' miRNA: 3'- -GCGUCAGGCGUUGGGCCCGGGAGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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