Results 81 - 84 of 84 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5204 | 5' | -60.5 | NC_001798.1 | + | 35279 | 0.66 | 0.707083 |
Target: 5'- aCGCGGg-CGgGGCUCGGGCUCUCc-- -3' miRNA: 3'- -GCGUCagGCgUUGGGCCCGGGAGuag -5' |
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5204 | 5' | -60.5 | NC_001798.1 | + | 52864 | 0.66 | 0.697333 |
Target: 5'- aGCgAGUCCGUgcuggGGCCgCGGGUCCgcgCGUg -3' miRNA: 3'- gCG-UCAGGCG-----UUGG-GCCCGGGa--GUAg -5' |
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5204 | 5' | -60.5 | NC_001798.1 | + | 101733 | 0.66 | 0.687532 |
Target: 5'- -aCAGaUCgGCGGCCCGGGCCgU-GUCc -3' miRNA: 3'- gcGUC-AGgCGUUGGGCCCGGgAgUAG- -5' |
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5204 | 5' | -60.5 | NC_001798.1 | + | 31996 | 0.66 | 0.73594 |
Target: 5'- gGCGGcgCCGCG--CCGGGCCC-CGg- -3' miRNA: 3'- gCGUCa-GGCGUugGGCCCGGGaGUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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