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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5205 | 3' | -53.2 | NC_001798.1 | + | 81712 | 0.66 | 0.956741 |
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Target: 5'- -gCUUUCUCCGCcGCcgugccgcgcgUGCgcaggucccgCCGGACg -3' miRNA: 3'- aaGAAAGAGGUGaCG-----------ACGaa--------GGCCUG- -5' |
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| 5205 | 3' | -53.2 | NC_001798.1 | + | 14089 | 0.68 | 0.891282 |
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Target: 5'- -----gCUCCAUaGCUGCUguaccCCGGGCa -3' miRNA: 3'- aagaaaGAGGUGaCGACGAa----GGCCUG- -5' |
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| 5205 | 3' | -53.2 | NC_001798.1 | + | 125592 | 0.7 | 0.790562 |
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Target: 5'- cUCUguUUCUCCaggcgcuccagguACUGCUGCacgUCGGACg -3' miRNA: 3'- aAGA--AAGAGG-------------UGACGACGaa-GGCCUG- -5' |
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| 5205 | 3' | -53.2 | NC_001798.1 | + | 6850 | 0.73 | 0.659107 |
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Target: 5'- ----cUC-CCGCUGCUGUguccUCCGGACg -3' miRNA: 3'- aagaaAGaGGUGACGACGa---AGGCCUG- -5' |
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| 5205 | 3' | -53.2 | NC_001798.1 | + | 85647 | 1.06 | 0.006031 |
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Target: 5'- uUUCUUUCUCCACUGCUGCUUCCGGACc -3' miRNA: 3'- -AAGAAAGAGGUGACGACGAAGGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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