Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5221 | 3' | -57.3 | NC_001798.1 | + | 64123 | 0.66 | 0.870033 |
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Target: 5'- cCCGacGCGUGGguaucuagGGGGUCgGugCUCGcGGg -3' miRNA: 3'- aGGU--CGCACC--------UCCUAGaCugGAGC-CC- -5' |
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| 5221 | 3' | -57.3 | NC_001798.1 | + | 12579 | 0.67 | 0.847436 |
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Target: 5'- cCCGGaUGUGGGGGAgacguaGGCgCUUGGGg -3' miRNA: 3'- aGGUC-GCACCUCCUaga---CUG-GAGCCC- -5' |
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| 5221 | 3' | -57.3 | NC_001798.1 | + | 31803 | 0.67 | 0.847436 |
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Target: 5'- cCgGGCGcGGAGGGag-GACC-CGGGg -3' miRNA: 3'- aGgUCGCaCCUCCUagaCUGGaGCCC- -5' |
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| 5221 | 3' | -57.3 | NC_001798.1 | + | 133757 | 0.67 | 0.846653 |
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Target: 5'- gCCcGCGUGGGGGcgCUGcuugcgcacgcacGCCUCcGGc -3' miRNA: 3'- aGGuCGCACCUCCuaGAC-------------UGGAGcCC- -5' |
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| 5221 | 3' | -57.3 | NC_001798.1 | + | 50572 | 0.67 | 0.831423 |
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Target: 5'- -gCGGgGUGGGGGGUgUGuGCCgUUGGGu -3' miRNA: 3'- agGUCgCACCUCCUAgAC-UGG-AGCCC- -5' |
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| 5221 | 3' | -57.3 | NC_001798.1 | + | 30756 | 0.67 | 0.831423 |
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Target: 5'- aCCcGCGUGGAGGccgaggcGGCCgugCGGGc -3' miRNA: 3'- aGGuCGCACCUCCuaga---CUGGa--GCCC- -5' |
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| 5221 | 3' | -57.3 | NC_001798.1 | + | 74952 | 1.1 | 0.002229 |
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Target: 5'- gUCCAGCGUGGAGGAUCUGACCUCGGGc -3' miRNA: 3'- -AGGUCGCACCUCCUAGACUGGAGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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