Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5221 | 3' | -57.3 | NC_001798.1 | + | 29677 | 0.69 | 0.722914 |
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Target: 5'- cCCGGCG-GGAGGAggaGACC-CGGa -3' miRNA: 3'- aGGUCGCaCCUCCUagaCUGGaGCCc -5' |
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| 5221 | 3' | -57.3 | NC_001798.1 | + | 93121 | 0.7 | 0.693519 |
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Target: 5'- cCCGGCG-GGcGGGUuugagcagcgcCUGGCCUCGGu -3' miRNA: 3'- aGGUCGCaCCuCCUA-----------GACUGGAGCCc -5' |
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| 5221 | 3' | -57.3 | NC_001798.1 | + | 139367 | 0.7 | 0.66368 |
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Target: 5'- gUCCGGCGgGGAGGGcUCgcggGACgUcCGGGc -3' miRNA: 3'- -AGGUCGCaCCUCCU-AGa---CUGgA-GCCC- -5' |
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| 5221 | 3' | -57.3 | NC_001798.1 | + | 109431 | 0.7 | 0.662681 |
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Target: 5'- cCCGGCGgcccgccugcggcUGGAGGcgC--GCCUCGGGc -3' miRNA: 3'- aGGUCGC-------------ACCUCCuaGacUGGAGCCC- -5' |
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| 5221 | 3' | -57.3 | NC_001798.1 | + | 41440 | 0.7 | 0.653674 |
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Target: 5'- gCgGGCGUGGGGGGUCcaUGcCCcgcCGGGg -3' miRNA: 3'- aGgUCGCACCUCCUAG--ACuGGa--GCCC- -5' |
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| 5221 | 3' | -57.3 | NC_001798.1 | + | 26533 | 0.73 | 0.496112 |
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Target: 5'- -gCGGCGUGGAGGuggugggGACCgccgCGGGg -3' miRNA: 3'- agGUCGCACCUCCuaga---CUGGa---GCCC- -5' |
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| 5221 | 3' | -57.3 | NC_001798.1 | + | 74952 | 1.1 | 0.002229 |
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Target: 5'- gUCCAGCGUGGAGGAUCUGACCUCGGGc -3' miRNA: 3'- -AGGUCGCACCUCCUAGACUGGAGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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