Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5228 | 5' | -55.9 | NC_001798.1 | + | 52557 | 0.66 | 0.888156 |
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Target: 5'- cCUGCAGCGGcUGcUGGGCc-UGG-CCGa -3' miRNA: 3'- -GACGUCGUCuGC-ACCCGaaACCaGGU- -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 13075 | 0.66 | 0.888156 |
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Target: 5'- uUGCAGgAGGCGcGGGCccggUGcUCCAg -3' miRNA: 3'- gACGUCgUCUGCaCCCGaa--ACcAGGU- -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 139556 | 0.66 | 0.894266 |
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Target: 5'- cCUGCAGUgguuccgGGACGUGGcggcCUUggaGGUCCc -3' miRNA: 3'- -GACGUCG-------UCUGCACCc---GAAa--CCAGGu -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 64502 | 0.66 | 0.894933 |
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Target: 5'- -cGcCGGaGGGgGUGGGCgg-GGUCCAg -3' miRNA: 3'- gaC-GUCgUCUgCACCCGaaaCCAGGU- -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 41840 | 0.66 | 0.90778 |
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Target: 5'- -aGC-GCAGACacgaGaGGCUUUGGUUCAg -3' miRNA: 3'- gaCGuCGUCUGca--C-CCGAAACCAGGU- -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 31254 | 0.66 | 0.90778 |
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Target: 5'- uUGCGGgCGGGgGUGGGaucugGGUCUg -3' miRNA: 3'- gACGUC-GUCUgCACCCgaaa-CCAGGu -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 87179 | 0.66 | 0.90778 |
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Target: 5'- gUGCAGCgcGGGCGacGGGCggcUGG-CCAc -3' miRNA: 3'- gACGUCG--UCUGCa-CCCGaa-ACCaGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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