Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5228 | 5' | -55.9 | NC_001798.1 | + | 96983 | 0.7 | 0.672124 |
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Target: 5'- -cGCGGCGGGCGgcgGGGCcagGG-CCGg -3' miRNA: 3'- gaCGUCGUCUGCa--CCCGaaaCCaGGU- -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 97840 | 0.67 | 0.826074 |
|
Target: 5'- cCUGaCGGCGGGCGcGGGCac-GGUCa- -3' miRNA: 3'- -GAC-GUCGUCUGCaCCCGaaaCCAGgu -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 118626 | 0.7 | 0.702793 |
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Target: 5'- gCUGCAGCGGuGCGaGGGUcgagUGGUCUu -3' miRNA: 3'- -GACGUCGUC-UGCaCCCGaa--ACCAGGu -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 125237 | 0.66 | 0.880434 |
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Target: 5'- gCUGUAGUAGGCGUugcguugGGGCacgGcGUCCu -3' miRNA: 3'- -GACGUCGUCUGCA-------CCCGaaaC-CAGGu -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 134471 | 0.68 | 0.781199 |
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Target: 5'- -cGCGGUuGGCGUGGGCgcgccUGGccgCCAu -3' miRNA: 3'- gaCGUCGuCUGCACCCGaa---ACCa--GGU- -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 139556 | 0.66 | 0.894266 |
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Target: 5'- cCUGCAGUgguuccgGGACGUGGcggcCUUggaGGUCCc -3' miRNA: 3'- -GACGUCG-------UCUGCACCc---GAAa--CCAGGu -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 152234 | 0.69 | 0.752563 |
|
Target: 5'- -cGCGGCGGcCGcucgGGGCcggGGUCCGc -3' miRNA: 3'- gaCGUCGUCuGCa---CCCGaaaCCAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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