Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5228 | 5' | -55.9 | NC_001798.1 | + | 20265 | 0.69 | 0.752563 |
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Target: 5'- uCUGCAGCAGgcggccccACGUGcgcggccccaGGCg--GGUCCGg -3' miRNA: 3'- -GACGUCGUC--------UGCAC----------CCGaaaCCAGGU- -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 15466 | 0.71 | 0.620456 |
|
Target: 5'- -cGCgugGGUAGACGUGGGCgggggGGUCg- -3' miRNA: 3'- gaCG---UCGUCUGCACCCGaaa--CCAGgu -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 15385 | 0.66 | 0.883978 |
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Target: 5'- -cGCGGCGGAggcgcccaccggaccCG-GGGUUgcgGGUCCGg -3' miRNA: 3'- gaCGUCGUCU---------------GCaCCCGAaa-CCAGGU- -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 13075 | 0.66 | 0.888156 |
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Target: 5'- uUGCAGgAGGCGcGGGCccggUGcUCCAg -3' miRNA: 3'- gACGUCgUCUGCaCCCGaa--ACcAGGU- -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 3019 | 0.66 | 0.873913 |
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Target: 5'- -cGCGGCGGGCc-GGGCUccGG-CCAg -3' miRNA: 3'- gaCGUCGUCUGcaCCCGAaaCCaGGU- -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 1964 | 0.66 | 0.873913 |
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Target: 5'- cCUGCGcGCccagcgccgAGACGUcggGGGCgc-GGUCCAg -3' miRNA: 3'- -GACGU-CG---------UCUGCA---CCCGaaaCCAGGU- -5' |
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| 5228 | 5' | -55.9 | NC_001798.1 | + | 420 | 0.66 | 0.879001 |
|
Target: 5'- -cGCGGCAGccccucccccccgcGCGccacgGGGCUgcGGUCCc -3' miRNA: 3'- gaCGUCGUC--------------UGCa----CCCGAaaCCAGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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