Results 101 - 104 of 104 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5241 | 3' | -58.4 | NC_001798.1 | + | 33390 | 0.66 | 0.839808 |
Target: 5'- gGUGGGCaggaguGGGAGGGCGccuGGCuCGGGGa- -3' miRNA: 3'- gCACCUG------CUCUUCCGC---CUG-GUCCCac -5' |
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5241 | 3' | -58.4 | NC_001798.1 | + | 20949 | 0.66 | 0.839808 |
Target: 5'- uCGUGu-UGAGGcGGGCGGGuCCGGGGg- -3' miRNA: 3'- -GCACcuGCUCU-UCCGCCU-GGUCCCac -5' |
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5241 | 3' | -58.4 | NC_001798.1 | + | 36267 | 0.66 | 0.839808 |
Target: 5'- uGUGGAcccCGGGGugggcGGCGGGggGGGGUGc -3' miRNA: 3'- gCACCU---GCUCUu----CCGCCUggUCCCAC- -5' |
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5241 | 3' | -58.4 | NC_001798.1 | + | 59540 | 0.66 | 0.847685 |
Target: 5'- --gGGACcccGGAAGGCGGAgaGGGGg- -3' miRNA: 3'- gcaCCUGc--UCUUCCGCCUggUCCCac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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