miRNA: 3'- gcACCUGCUCuuCCGC-CUGg----UCCCac -5'
miRNA: 3'- -GCACCU--------------GCU--CuuCC-GCCUGGUCCCac -5'
miRNA: 3'- gCAcCUG----CUCUUCCGCC-UGGUCCCac -5'
miRNA: 3'- gCA-CCUGCUCU---UCCGCC-------------UGGUCCCac -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5241 | 3' | -58.4 | NC_001798.1 | + | 14989 | 0.68 | 0.74293 |
|
Target: 5'- -uUGGGCGGGggGGCGaGGCguguuGGGGg- -3' miRNA: 3'- gcACCUGCUCuuCCGC-CUGg----UCCCac -5' |
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| 5241 | 3' | -58.4 | NC_001798.1 | + | 151589 | 0.68 | 0.74008 |
|
Target: 5'- gCGUGGGgcugcccuggcgcuCGGccGggGGcCGGGCCGGGGg- -3' miRNA: 3'- -GCACCU--------------GCU--CuuCC-GCCUGGUCCCac -5' |
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| 5241 | 3' | -58.4 | NC_001798.1 | + | 124565 | 0.68 | 0.7334 |
|
Target: 5'- gGUcGAUcucgGGGAGGGCGGcACCGGGGa- -3' miRNA: 3'- gCAcCUG----CUCUUCCGCC-UGGUCCCac -5' |
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| 5241 | 3' | -58.4 | NC_001798.1 | + | 31044 | 0.69 | 0.673774 |
|
Target: 5'- gGUcGGGCGGGGgucGGGCGGgggucgggcacuaACCGGGGg- -3' miRNA: 3'- gCA-CCUGCUCU---UCCGCC-------------UGGUCCCac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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