Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5241 | 5' | -57.6 | NC_001798.1 | + | 127968 | 0.66 | 0.861059 |
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Target: 5'- cGCGUCCC--CgGCCcUCUCUGaGGCc -3' miRNA: 3'- uCGCAGGGcaGgCGGaAGAGACaCUG- -5' |
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| 5241 | 5' | -57.6 | NC_001798.1 | + | 105254 | 0.66 | 0.852636 |
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Target: 5'- cGGCGUCCCGcgcggcaUCCGCCUcUUCggccGcGGCc -3' miRNA: 3'- -UCGCAGGGC-------AGGCGGAaGAGa---CaCUG- -5' |
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| 5241 | 5' | -57.6 | NC_001798.1 | + | 148014 | 0.66 | 0.837534 |
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Target: 5'- uGGCG-CCCGUCC-CCUUcCUCUaccgcGUGGg -3' miRNA: 3'- -UCGCaGGGCAGGcGGAA-GAGA-----CACUg -5' |
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| 5241 | 5' | -57.6 | NC_001798.1 | + | 106720 | 0.67 | 0.820927 |
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Target: 5'- cGGCGUCCCGUCCuaGCCaUCgccagGgGGCc -3' miRNA: 3'- -UCGCAGGGCAGG--CGGaAGaga--CaCUG- -5' |
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| 5241 | 5' | -57.6 | NC_001798.1 | + | 124094 | 0.67 | 0.815811 |
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Target: 5'- uGCGcCCCGacggcccacaucgauUCCGCCaaCgcgCUGUGGCg -3' miRNA: 3'- uCGCaGGGC---------------AGGCGGaaGa--GACACUG- -5' |
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| 5241 | 5' | -57.6 | NC_001798.1 | + | 87932 | 0.67 | 0.812367 |
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Target: 5'- uGGCGUaCCCGggauccUCCGCCagCUCg--GACa -3' miRNA: 3'- -UCGCA-GGGC------AGGCGGaaGAGacaCUG- -5' |
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| 5241 | 5' | -57.6 | NC_001798.1 | + | 5960 | 0.67 | 0.801002 |
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Target: 5'- cGGCGUCCCGgucgCCGCCgcaccagggGACa -3' miRNA: 3'- -UCGCAGGGCa---GGCGGaagagaca-CUG- -5' |
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| 5241 | 5' | -57.6 | NC_001798.1 | + | 21644 | 0.67 | 0.785761 |
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Target: 5'- cGGUGcCCCGcucgCCGCCggcgUCUCUGUc-- -3' miRNA: 3'- -UCGCaGGGCa---GGCGGa---AGAGACAcug -5' |
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| 5241 | 5' | -57.6 | NC_001798.1 | + | 23113 | 0.68 | 0.767333 |
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Target: 5'- aAGCGgCCCGcUCGCCUUCUCcGccgcgGACc -3' miRNA: 3'- -UCGCaGGGCaGGCGGAAGAGaCa----CUG- -5' |
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| 5241 | 5' | -57.6 | NC_001798.1 | + | 64378 | 1.1 | 0.001766 |
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Target: 5'- gAGCGUCCCGUCCGCCUUCUCUGUGACa -3' miRNA: 3'- -UCGCAGGGCAGGCGGAAGAGACACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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