Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5256 | 3' | -55.8 | NC_001798.1 | + | 24992 | 0.67 | 0.871544 |
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Target: 5'- ----cCCUGCUGGCCGacaccGUCGcCGCGg -3' miRNA: 3'- cuuaaGGACGGUCGGCga---CAGC-GUGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 133023 | 0.67 | 0.878829 |
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Target: 5'- gGAcgUCgC-GCCGGCCGaggugGUCGCGCu -3' miRNA: 3'- -CUuaAG-GaCGGUCGGCga---CAGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 135761 | 0.66 | 0.885891 |
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Target: 5'- cGAAUccUCCggcgGCCGGCCGCcGgccCGC-CGg -3' miRNA: 3'- -CUUA--AGGa---CGGUCGGCGaCa--GCGuGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 84791 | 0.66 | 0.885891 |
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Target: 5'- aGGAgggagGCCAGCCGCUG-CGUccGCGa -3' miRNA: 3'- -CUUaaggaCGGUCGGCGACaGCG--UGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 1356 | 0.66 | 0.892725 |
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Target: 5'- ---cUCC-GCCgGGCCGCcG-CGCACGg -3' miRNA: 3'- cuuaAGGaCGG-UCGGCGaCaGCGUGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 28242 | 0.66 | 0.899327 |
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Target: 5'- ----gCCUGCCccccGCCGCcGcCGCGCa -3' miRNA: 3'- cuuaaGGACGGu---CGGCGaCaGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 4593 | 0.66 | 0.903808 |
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Target: 5'- aGAAggCgaGCgGGCCGCUucuucuugcgcgccGUCGCGCu -3' miRNA: 3'- -CUUaaGgaCGgUCGGCGA--------------CAGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 75728 | 0.66 | 0.91182 |
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Target: 5'- ----cCCcGCCGGCCaauCUGUaCGCACGc -3' miRNA: 3'- cuuaaGGaCGGUCGGc--GACA-GCGUGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 128156 | 0.66 | 0.91182 |
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Target: 5'- ----aCCUGCgCGGCCuGCUG-CGCgGCGu -3' miRNA: 3'- cuuaaGGACG-GUCGG-CGACaGCG-UGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 139039 | 0.66 | 0.91182 |
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Target: 5'- ----aCCUGCgaacCAGCCGCUG-CgGCugGa -3' miRNA: 3'- cuuaaGGACG----GUCGGCGACaG-CGugC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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