Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5256 | 3' | -55.8 | NC_001798.1 | + | 103493 | 0.7 | 0.709181 |
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Target: 5'- ---gUCCaggGCCAGCucgcccgggucccCGCUGUCGUACa -3' miRNA: 3'- cuuaAGGa--CGGUCG-------------GCGACAGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 71615 | 0.7 | 0.700086 |
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Target: 5'- cGGcgUCCUcaGCCAGaCGCUGgCGCGCa -3' miRNA: 3'- -CUuaAGGA--CGGUCgGCGACaGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 39078 | 0.7 | 0.689926 |
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Target: 5'- --uUUCCUGgCgCAGCaCGCaGUCGCGCa -3' miRNA: 3'- cuuAAGGAC-G-GUCG-GCGaCAGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 94157 | 0.71 | 0.659189 |
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Target: 5'- ----aCCacGCCAuGUCGCUGUCGCugGa -3' miRNA: 3'- cuuaaGGa-CGGU-CGGCGACAGCGugC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 135069 | 0.72 | 0.597341 |
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Target: 5'- ---cUCCUGCCcGCCuuucgggaaGCgGUCGCGCGg -3' miRNA: 3'- cuuaAGGACGGuCGG---------CGaCAGCGUGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 71819 | 0.72 | 0.576851 |
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Target: 5'- ----cCCgGCCAcGCCGCUGUcCGCGCu -3' miRNA: 3'- cuuaaGGaCGGU-CGGCGACA-GCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 121802 | 0.72 | 0.566663 |
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Target: 5'- ----gCCggGCCGGCCGUUGUCgGCACc -3' miRNA: 3'- cuuaaGGa-CGGUCGGCGACAG-CGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 113577 | 0.73 | 0.526438 |
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Target: 5'- ----gCCaGCCAGCUGCUGcccCGCACGg -3' miRNA: 3'- cuuaaGGaCGGUCGGCGACa--GCGUGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 94368 | 0.74 | 0.477769 |
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Target: 5'- cGggUUCCUGUCGGCgaagaCGCUgacgGUCGCGCu -3' miRNA: 3'- -CuuAAGGACGGUCG-----GCGA----CAGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 55843 | 1.09 | 0.002655 |
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Target: 5'- uGAAUUCCUGCCAGCCGCUGUCGCACGg -3' miRNA: 3'- -CUUAAGGACGGUCGGCGACAGCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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