Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5256 | 3' | -55.8 | NC_001798.1 | + | 103493 | 0.7 | 0.709181 |
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Target: 5'- ---gUCCaggGCCAGCucgcccgggucccCGCUGUCGUACa -3' miRNA: 3'- cuuaAGGa--CGGUCG-------------GCGACAGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 113577 | 0.73 | 0.526438 |
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Target: 5'- ----gCCaGCCAGCUGCUGcccCGCACGg -3' miRNA: 3'- cuuaaGGaCGGUCGGCGACa--GCGUGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 121802 | 0.72 | 0.566663 |
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Target: 5'- ----gCCggGCCGGCCGUUGUCgGCACc -3' miRNA: 3'- cuuaaGGa-CGGUCGGCGACAG-CGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 124114 | 0.68 | 0.787784 |
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Target: 5'- cGAUUCC-GCCAaCgCGCUGUgGCGCGc -3' miRNA: 3'- cUUAAGGaCGGUcG-GCGACAgCGUGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 128156 | 0.66 | 0.91182 |
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Target: 5'- ----aCCUGCgCGGCCuGCUG-CGCgGCGu -3' miRNA: 3'- cuuaaGGACG-GUCGG-CGACaGCG-UGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 133023 | 0.67 | 0.878829 |
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Target: 5'- gGAcgUCgC-GCCGGCCGaggugGUCGCGCu -3' miRNA: 3'- -CUuaAG-GaCGGUCGGCga---CAGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 134372 | 0.68 | 0.805945 |
|
Target: 5'- ----gCCcGCCGGCCGCUccUCGCGCc -3' miRNA: 3'- cuuaaGGaCGGUCGGCGAc-AGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 135069 | 0.72 | 0.597341 |
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Target: 5'- ---cUCCUGCCcGCCuuucgggaaGCgGUCGCGCGg -3' miRNA: 3'- cuuaAGGACGGuCGG---------CGaCAGCGUGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 135761 | 0.66 | 0.885891 |
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Target: 5'- cGAAUccUCCggcgGCCGGCCGCcGgccCGC-CGg -3' miRNA: 3'- -CUUA--AGGa---CGGUCGGCGaCa--GCGuGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 139039 | 0.66 | 0.91182 |
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Target: 5'- ----aCCUGCgaacCAGCCGCUG-CgGCugGa -3' miRNA: 3'- cuuaaGGACG----GUCGGCGACaG-CGugC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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