Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5256 | 3' | -55.8 | NC_001798.1 | + | 52869 | 0.67 | 0.871544 |
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Target: 5'- ---gUCCgUGCUggGGCCGCggGUCcGCGCGu -3' miRNA: 3'- cuuaAGG-ACGG--UCGGCGa-CAG-CGUGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 51932 | 0.67 | 0.848402 |
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Target: 5'- uGGGggCCgccGCCGuGCCGCUGcUCaGCGCGg -3' miRNA: 3'- -CUUaaGGa--CGGU-CGGCGAC-AG-CGUGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 51465 | 0.68 | 0.823466 |
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Target: 5'- aGAUUCUcGCgGGCCGCgUGUCGgGCc -3' miRNA: 3'- cUUAAGGaCGgUCGGCG-ACAGCgUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 39078 | 0.7 | 0.689926 |
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Target: 5'- --uUUCCUGgCgCAGCaCGCaGUCGCGCa -3' miRNA: 3'- cuuAAGGAC-G-GUCG-GCGaCAGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 28242 | 0.66 | 0.899327 |
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Target: 5'- ----gCCUGCCccccGCCGCcGcCGCGCa -3' miRNA: 3'- cuuaaGGACGGu---CGGCGaCaGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 24992 | 0.67 | 0.871544 |
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Target: 5'- ----cCCUGCUGGCCGacaccGUCGcCGCGg -3' miRNA: 3'- cuuaaGGACGGUCGGCga---CAGC-GUGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 5179 | 0.68 | 0.796941 |
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Target: 5'- ---aUCCUGgCGGCCGCgucgGUagcCGCGCu -3' miRNA: 3'- cuuaAGGACgGUCGGCGa---CA---GCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 4593 | 0.66 | 0.903808 |
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Target: 5'- aGAAggCgaGCgGGCCGCUucuucuugcgcgccGUCGCGCu -3' miRNA: 3'- -CUUaaGgaCGgUCGGCGA--------------CAGCGUGc -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 3592 | 0.69 | 0.740052 |
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Target: 5'- -----gCUGCCggagaugaaggAGCUGCUGUUGCGCGc -3' miRNA: 3'- cuuaagGACGG-----------UCGGCGACAGCGUGC- -5' |
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| 5256 | 3' | -55.8 | NC_001798.1 | + | 1356 | 0.66 | 0.892725 |
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Target: 5'- ---cUCC-GCCgGGCCGCcG-CGCACGg -3' miRNA: 3'- cuuaAGGaCGG-UCGGCGaCaGCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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