miRNA: 3'- -GGgUGCuCCUGGCuCC-CCCa-UGCUCU- -5'
miRNA: 3'- -GGGUGcuCCU-GGCUCC----CCCAuGCUCU- -5'
miRNA: 3'- -GGGUGCUCCU--GGCUCCccca--UGCU-CU- -5'
miRNA: 3'- gGGUG-----CUC-CUGGCUC-CCCCa------------UGCUCU- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5256 | 5' | -58.8 | NC_001798.1 | + | 96971 | 0.66 | 0.852342 |
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Target: 5'- gCUgGCGcGGACCGcGGcGGGcgGCGGGGc -3' miRNA: 3'- -GGgUGCuCCUGGCuCC-CCCa-UGCUCU- -5' |
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| 5256 | 5' | -58.8 | NC_001798.1 | + | 15397 | 0.66 | 0.852342 |
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Target: 5'- gCCCACc-GGAcCCGGGGuugcGGGUcCGGGAg -3' miRNA: 3'- -GGGUGcuCCU-GGCUCC----CCCAuGCUCU- -5' |
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| 5256 | 5' | -58.8 | NC_001798.1 | + | 108697 | 0.66 | 0.852342 |
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Target: 5'- gCCCGCGGGGAcaCCGAGGcauccgACGAcGAa -3' miRNA: 3'- -GGGUGCUCCU--GGCUCCccca--UGCU-CU- -5' |
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| 5256 | 5' | -58.8 | NC_001798.1 | + | 146277 | 0.65 | 0.856846 |
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Target: 5'- aCCGCagccgGAGaGGCCGAGcGGGGagugggcggccggGCGGGAg -3' miRNA: 3'- gGGUG-----CUC-CUGGCUC-CCCCa------------UGCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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