miRNA: 3'- -GGGUGCUCCU---------GGCU--------CCCCCAugCUCU- -5'
miRNA: 3'- -GGGUG------CUCCUG-GCUCCCCCAUGCU-CU- -5'
miRNA: 3'- -GGGUGCUCCUGGCUCCCCca--UGCUCu -5'
miRNA: 3'- -GGGUGCUCCUgGCUCCCCCAUGCUCU- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5256 | 5' | -58.8 | NC_001798.1 | + | 137444 | 0.78 | 0.231905 |
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Target: 5'- cCCCGCGGGGAcgcgcucgcCCGGaaaucggcGGGGGUugGGGAg -3' miRNA: 3'- -GGGUGCUCCU---------GGCU--------CCCCCAugCUCU- -5' |
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| 5256 | 5' | -58.8 | NC_001798.1 | + | 126602 | 0.79 | 0.196483 |
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Target: 5'- gCCCGCagcuucGGGGGCuCGGGGGGGUACGGcGAg -3' miRNA: 3'- -GGGUG------CUCCUG-GCUCCCCCAUGCU-CU- -5' |
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| 5256 | 5' | -58.8 | NC_001798.1 | + | 16179 | 0.83 | 0.114312 |
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Target: 5'- gCCCGCGGGGACCGGGGGGacgcACGGGc -3' miRNA: 3'- -GGGUGCUCCUGGCUCCCCca--UGCUCu -5' |
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| 5256 | 5' | -58.8 | NC_001798.1 | + | 55878 | 0.98 | 0.011144 |
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Target: 5'- cCCCACGAGGA-CGAGGGGGUACGAGAa -3' miRNA: 3'- -GGGUGCUCCUgGCUCCCCCAUGCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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