Results 101 - 104 of 104 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5256 | 5' | -58.8 | NC_001798.1 | + | 152065 | 0.67 | 0.79872 |
Target: 5'- gCCCACGGGGGucccCCGAccgcuuaagcgggccGGGGGU-CGGc- -3' miRNA: 3'- -GGGUGCUCCU----GGCU---------------CCCCCAuGCUcu -5' |
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5256 | 5' | -58.8 | NC_001798.1 | + | 152516 | 0.67 | 0.778501 |
Target: 5'- cCCgGCGGGGGCgGAGGGaGGgaaucccccccucuCGGGGc -3' miRNA: 3'- -GGgUGCUCCUGgCUCCC-CCau------------GCUCU- -5' |
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5256 | 5' | -58.8 | NC_001798.1 | + | 152821 | 0.66 | 0.828855 |
Target: 5'- -aCGCGGGGAgCGAGGGGccGUcCGAa- -3' miRNA: 3'- ggGUGCUCCUgGCUCCCC--CAuGCUcu -5' |
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5256 | 5' | -58.8 | NC_001798.1 | + | 154356 | 0.69 | 0.653069 |
Target: 5'- cCCC-CGGGGAgucaacccCCGGGGGGGguguuuuUugGGGGg -3' miRNA: 3'- -GGGuGCUCCU--------GGCUCCCCC-------AugCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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