Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5257 | 3' | -53.2 | NC_001798.1 | + | 95301 | 0.66 | 0.971964 |
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Target: 5'- uUugUGuGCGCgg-CGuCGAGUCUCGGg -3' miRNA: 3'- gGugAC-UGCGacaGCuGCUCAGAGUC- -5' |
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| 5257 | 3' | -53.2 | NC_001798.1 | + | 98130 | 0.67 | 0.96906 |
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Target: 5'- gCGCUGAUuggGCcGUCaGCGAGUUUCAa -3' miRNA: 3'- gGUGACUG---CGaCAGcUGCUCAGAGUc -5' |
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| 5257 | 3' | -53.2 | NC_001798.1 | + | 80311 | 0.67 | 0.965949 |
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Target: 5'- cCCGCUGGCGCccUGgcgcUCGGCcuugGAG-CUCGGg -3' miRNA: 3'- -GGUGACUGCG--AC----AGCUG----CUCaGAGUC- -5' |
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| 5257 | 3' | -53.2 | NC_001798.1 | + | 54390 | 0.67 | 0.965949 |
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Target: 5'- gCCGC-GGCGCgGUCGGCGGGagCUgcCGGg -3' miRNA: 3'- -GGUGaCUGCGaCAGCUGCUCa-GA--GUC- -5' |
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| 5257 | 3' | -53.2 | NC_001798.1 | + | 49709 | 0.67 | 0.962624 |
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Target: 5'- -aGCUGGCGCcaGUUGGCGugucuGUUUCAGc -3' miRNA: 3'- ggUGACUGCGa-CAGCUGCu----CAGAGUC- -5' |
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| 5257 | 3' | -53.2 | NC_001798.1 | + | 49030 | 0.68 | 0.932969 |
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Target: 5'- uCCGCUGuACGCcaccgGUCGGCuGAGUCa--- -3' miRNA: 3'- -GGUGAC-UGCGa----CAGCUG-CUCAGaguc -5' |
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| 5257 | 3' | -53.2 | NC_001798.1 | + | 38792 | 0.69 | 0.922358 |
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Target: 5'- gCCGCgcucGACGCgGUUGGCGAGg--CGGg -3' miRNA: 3'- -GGUGa---CUGCGaCAGCUGCUCagaGUC- -5' |
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| 5257 | 3' | -53.2 | NC_001798.1 | + | 19498 | 0.7 | 0.891638 |
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Target: 5'- cCCGCUGGcCGUUGgCGACGAGUggcCUCc- -3' miRNA: 3'- -GGUGACU-GCGACaGCUGCUCA---GAGuc -5' |
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| 5257 | 3' | -53.2 | NC_001798.1 | + | 15112 | 0.73 | 0.748848 |
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Target: 5'- gCCGgUGGCGCUGUCGuCGuc-CUCGGg -3' miRNA: 3'- -GGUgACUGCGACAGCuGCucaGAGUC- -5' |
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| 5257 | 3' | -53.2 | NC_001798.1 | + | 55634 | 1.11 | 0.004474 |
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Target: 5'- gCCACUGACGCUGUCGACGAGUCUCAGa -3' miRNA: 3'- -GGUGACUGCGACAGCUGCUCAGAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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