Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5282 | 5' | -54.5 | NC_001798.1 | + | 66240 | 0.66 | 0.943957 |
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Target: 5'- aGCUgggCCUCGUgUUCgcgaaGCCUUUCCGUCa -3' miRNA: 3'- cUGA---GGGGUAgAAGa----CGGGAAGGUAG- -5' |
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| 5282 | 5' | -54.5 | NC_001798.1 | + | 71191 | 0.66 | 0.939244 |
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Target: 5'- cGCUggCCAUCUUCcGUCCggCCGUCa -3' miRNA: 3'- cUGAggGGUAGAAGaCGGGaaGGUAG- -5' |
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| 5282 | 5' | -54.5 | NC_001798.1 | + | 5513 | 0.66 | 0.934284 |
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Target: 5'- cGCUCCCC------UGCCCUUCCGg- -3' miRNA: 3'- cUGAGGGGuagaagACGGGAAGGUag -5' |
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| 5282 | 5' | -54.5 | NC_001798.1 | + | 133254 | 0.66 | 0.923616 |
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Target: 5'- cGC-CCCCGUCUgggaGCUgUUCCGUCu -3' miRNA: 3'- cUGaGGGGUAGAaga-CGGgAAGGUAG- -5' |
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| 5282 | 5' | -54.5 | NC_001798.1 | + | 85395 | 0.66 | 0.917908 |
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Target: 5'- gGACcgCCCCuGUCUggUCUGCCgcaCCAUCg -3' miRNA: 3'- -CUGa-GGGG-UAGA--AGACGGgaaGGUAG- -5' |
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| 5282 | 5' | -54.5 | NC_001798.1 | + | 134139 | 0.68 | 0.847633 |
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Target: 5'- cGGCUCCCCcgacGUC-UCgGCCCccggggCCGUCg -3' miRNA: 3'- -CUGAGGGG----UAGaAGaCGGGaa----GGUAG- -5' |
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| 5282 | 5' | -54.5 | NC_001798.1 | + | 101459 | 0.73 | 0.592874 |
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Target: 5'- -cCUCCCCgugguaGUCUUCcgGgCCUUCCGUCg -3' miRNA: 3'- cuGAGGGG------UAGAAGa-CgGGAAGGUAG- -5' |
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| 5282 | 5' | -54.5 | NC_001798.1 | + | 35880 | 1.08 | 0.003828 |
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Target: 5'- gGACUCCCCAUCUUCUGCCCUUCCAUCc -3' miRNA: 3'- -CUGAGGGGUAGAAGACGGGAAGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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