Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5289 | 3' | -57 | NC_001798.1 | + | 132499 | 0.66 | 0.880944 |
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Target: 5'- gGGGcGGGUacggacucuccgcaAGGGGGAGCUgcugaucuacauGgacggguccggggcGCGCUCGg -3' miRNA: 3'- gCCC-CUCA--------------UCCCCCUCGA------------U--------------UGUGAGC- -5' |
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| 5289 | 3' | -57 | NC_001798.1 | + | 21561 | 0.66 | 0.885084 |
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Target: 5'- gGGGGGGgggGGGGGGAGggAAagGCa-- -3' miRNA: 3'- gCCCCUCa--UCCCCCUCgaUUg-UGagc -5' |
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| 5289 | 3' | -57 | NC_001798.1 | + | 73944 | 0.66 | 0.885084 |
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Target: 5'- uGGuGGAG-GGGGGcGAGUgGACGCgcgCGg -3' miRNA: 3'- gCC-CCUCaUCCCC-CUCGaUUGUGa--GC- -5' |
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| 5289 | 3' | -57 | NC_001798.1 | + | 14318 | 0.66 | 0.891807 |
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Target: 5'- uGGGGGGUGcGuGGGGAGgCguGCGgUCGc -3' miRNA: 3'- gCCCCUCAU-C-CCCCUC-GauUGUgAGC- -5' |
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| 5289 | 3' | -57 | NC_001798.1 | + | 27284 | 0.66 | 0.891807 |
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Target: 5'- gCGGGGGGgAGGGGcuGCcgcgaGCUCGg -3' miRNA: 3'- -GCCCCUCaUCCCCcuCGauug-UGAGC- -5' |
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| 5289 | 3' | -57 | NC_001798.1 | + | 27321 | 0.66 | 0.891807 |
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Target: 5'- aGGGGAGggAGGGGGuGGCggggAAC-CgugugCGg -3' miRNA: 3'- gCCCCUCa-UCCCCC-UCGa---UUGuGa----GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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