Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5290 | 5' | -54.1 | NC_001798.1 | + | 96343 | 0.66 | 0.954422 |
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Target: 5'- cGCGUGC-UGCGgcggcaccugcgGCCGGGgcugGAgGCUCa -3' miRNA: 3'- -UGCACGuACGCa-----------CGGCUCa---CU-UGAGa -5' |
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| 5290 | 5' | -54.1 | NC_001798.1 | + | 36310 | 0.66 | 0.942314 |
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Target: 5'- gGCG-GCGggGCGcggGCCGGGgccgGGGCUCg -3' miRNA: 3'- -UGCaCGUa-CGCa--CGGCUCa---CUUGAGa -5' |
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| 5290 | 5' | -54.1 | NC_001798.1 | + | 97714 | 0.67 | 0.921686 |
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Target: 5'- gGCGcUGCGUGCGcgagcaGCgCGGG-GAGCUCg -3' miRNA: 3'- -UGC-ACGUACGCa-----CG-GCUCaCUUGAGa -5' |
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| 5290 | 5' | -54.1 | NC_001798.1 | + | 117978 | 0.67 | 0.91591 |
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Target: 5'- gACGUGCAguuuaaGCGcccGCCGGGgugccgcGAGCUCg -3' miRNA: 3'- -UGCACGUa-----CGCa--CGGCUCa------CUUGAGa -5' |
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| 5290 | 5' | -54.1 | NC_001798.1 | + | 128668 | 0.7 | 0.810818 |
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Target: 5'- aACGUGCAcGCGUGCgGGGUc--CUCa -3' miRNA: 3'- -UGCACGUaCGCACGgCUCAcuuGAGa -5' |
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| 5290 | 5' | -54.1 | NC_001798.1 | + | 27831 | 1.07 | 0.005578 |
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Target: 5'- cACGUGCAUGCGUGCCGAGUGAACUCUc -3' miRNA: 3'- -UGCACGUACGCACGGCUCACUUGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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