Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5307 | 3' | -52.2 | NC_001798.1 | + | 12891 | 0.66 | 0.990744 |
Target: 5'- gGGUGCugGUGUACGgggGcUugGGgCCGUGCc -3' miRNA: 3'- -CCAUG--CACAUGCa--C-AugCUgGGCGUG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 102882 | 0.66 | 0.990744 |
Target: 5'- aGGUACGUGUguccgGCGauccCGGCCUGC-Cg -3' miRNA: 3'- -CCAUGCACA-----UGCacauGCUGGGCGuG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 135334 | 0.66 | 0.990622 |
Target: 5'- uGGUGCcgg-ACGcGUGCGAcgccugcCCCGCGCu -3' miRNA: 3'- -CCAUGcacaUGCaCAUGCU-------GGGCGUG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 13360 | 0.66 | 0.98999 |
Target: 5'- aGGU---UGUACGUGUGCuuucgggccucguccGAagcCCCGCGCa -3' miRNA: 3'- -CCAugcACAUGCACAUG---------------CU---GGGCGUG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 144741 | 0.66 | 0.988041 |
Target: 5'- --gACGgGUGCGUGgcugACGGCCUGUu- -3' miRNA: 3'- ccaUGCaCAUGCACa---UGCUGGGCGug -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 134633 | 0.66 | 0.984764 |
Target: 5'- uGGUcgAgGUGUGCGagGU-CGGCCUGCGg -3' miRNA: 3'- -CCA--UgCACAUGCa-CAuGCUGGGCGUg -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 143386 | 0.67 | 0.982889 |
Target: 5'- --cACGUGcGCGUccgGUGCGGucCCCGCAa -3' miRNA: 3'- ccaUGCACaUGCA---CAUGCU--GGGCGUg -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 153759 | 0.67 | 0.982889 |
Target: 5'- aGGUgggGCGgaagGggGCGc-UGCGGCCCGCGCu -3' miRNA: 3'- -CCA---UGCa---Ca-UGCacAUGCUGGGCGUG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 14940 | 0.67 | 0.982889 |
Target: 5'- aGGgggAUGUGUugGggUGUGgGGCCCGagGCa -3' miRNA: 3'- -CCa--UGCACAugC--ACAUgCUGGGCg-UG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 131122 | 0.67 | 0.982092 |
Target: 5'- cGUACGUGgccagcaggcucUGCGUGUccaaccggagggggACGGcuacaccCCCGCGCa -3' miRNA: 3'- cCAUGCAC------------AUGCACA--------------UGCU-------GGGCGUG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 92960 | 0.67 | 0.976217 |
Target: 5'- -cUGCGUGcccggaACGUGgACGccgugGCCCGCGCg -3' miRNA: 3'- ccAUGCACa-----UGCACaUGC-----UGGGCGUG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 108861 | 0.68 | 0.967812 |
Target: 5'- cGGgcugGCGUu--CGgGUGCGACgCCGCGCu -3' miRNA: 3'- -CCa---UGCAcauGCaCAUGCUG-GGCGUG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 121556 | 0.68 | 0.964592 |
Target: 5'- --gGCGUGgggccCGUGgGCGACCUGUGCc -3' miRNA: 3'- ccaUGCACau---GCACaUGCUGGGCGUG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 74295 | 0.68 | 0.957491 |
Target: 5'- aGGUGCGggcgGgcgACGUGU---ACCCGUACc -3' miRNA: 3'- -CCAUGCa---Ca--UGCACAugcUGGGCGUG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 37139 | 0.68 | 0.957491 |
Target: 5'- aGGUGUGUGaGCGUGgcCG-CUCGCGCc -3' miRNA: 3'- -CCAUGCACaUGCACauGCuGGGCGUG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 119518 | 0.69 | 0.94511 |
Target: 5'- gGGgcagGCGUGUugGgaaGgGACCCGCGg -3' miRNA: 3'- -CCa---UGCACAugCacaUgCUGGGCGUg -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 120780 | 0.69 | 0.94511 |
Target: 5'- cGUugGUGUGCGUc-ACcACCCGCGg -3' miRNA: 3'- cCAugCACAUGCAcaUGcUGGGCGUg -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 83508 | 0.7 | 0.907779 |
Target: 5'- --aGCGuUGUugGUGgccaacACGAgCCCGCGCa -3' miRNA: 3'- ccaUGC-ACAugCACa-----UGCU-GGGCGUG- -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 140205 | 0.71 | 0.895597 |
Target: 5'- cGGUGCugGUGUGCGUGgggucgccgacccagACGGCCuCGCu- -3' miRNA: 3'- -CCAUG--CACAUGCACa--------------UGCUGG-GCGug -5' |
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5307 | 3' | -52.2 | NC_001798.1 | + | 92252 | 0.71 | 0.888155 |
Target: 5'- gGGgccgAUGggGUACGUGUAUGG-CCGCGCc -3' miRNA: 3'- -CCa---UGCa-CAUGCACAUGCUgGGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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