Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5313 | 5' | -55 | NC_001798.1 | + | 44643 | 0.71 | 0.796318 |
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Target: 5'- gCCAUugGGCGcauCGGACCGguacgCGUCGUa- -3' miRNA: 3'- -GGUA--CCGCau-GCCUGGCa----GCAGCAcc -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 24164 | 0.74 | 0.63302 |
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Target: 5'- gCCAUGGCG-GCGGGCCG-CuUCGgcugGGg -3' miRNA: 3'- -GGUACCGCaUGCCUGGCaGcAGCa---CC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 15921 | 0.74 | 0.613032 |
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Target: 5'- ---gGGCGUACGGACCGUCaUCuagGGc -3' miRNA: 3'- gguaCCGCAUGCCUGGCAGcAGca-CC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 36835 | 0.74 | 0.603055 |
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Target: 5'- ---cGGCGUGgGGACCGgcugUGUgGUGGg -3' miRNA: 3'- gguaCCGCAUgCCUGGCa---GCAgCACC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 32556 | 0.75 | 0.592103 |
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Target: 5'- aCGUGGCGgaccugcUGCGGGCCG-CGgcccccgCGUGGa -3' miRNA: 3'- gGUACCGC-------AUGCCUGGCaGCa------GCACC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 51598 | 0.75 | 0.583168 |
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Target: 5'- cCCGUGuccggauagcGCGUuCGGGCUGUCGcgCGUGGg -3' miRNA: 3'- -GGUAC----------CGCAuGCCUGGCAGCa-GCACC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 3361 | 0.76 | 0.534145 |
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Target: 5'- gUCGUGGCGguugGCGucgccGCCGUCGUCGgGGg -3' miRNA: 3'- -GGUACCGCa---UGCc----UGGCAGCAGCaCC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 19171 | 0.76 | 0.504485 |
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Target: 5'- aCCGUGGCcccugcccgaucgGUuuuugcccgaGCGGACCGU-GUCGUGGa -3' miRNA: 3'- -GGUACCG-------------CA----------UGCCUGGCAgCAGCACC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 10134 | 1.13 | 0.002902 |
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Target: 5'- uCCAUGGCGUACGGACCGUCGUCGUGGg -3' miRNA: 3'- -GGUACCGCAUGCCUGGCAGCAGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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