Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5320 | 5' | -48.6 | NC_001798.1 | + | 148398 | 0.66 | 0.997011 |
Target: 5'- ------cGGGCAGGuGUGCGGGCGg-- -3' miRNA: 3'- uaauaauCCCGUUU-CACGCUCGUgac -5' |
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5320 | 5' | -48.6 | NC_001798.1 | + | 148426 | 0.7 | 0.969541 |
Target: 5'- -----cGGGGCGGGG-GUGGGCACg- -3' miRNA: 3'- uaauaaUCCCGUUUCaCGCUCGUGac -5' |
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5320 | 5' | -48.6 | NC_001798.1 | + | 148443 | 0.69 | 0.982684 |
Target: 5'- ------cGGGCGuAAGUGCGGGUGCa- -3' miRNA: 3'- uaauaauCCCGU-UUCACGCUCGUGac -5' |
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5320 | 5' | -48.6 | NC_001798.1 | + | 148510 | 0.68 | 0.989673 |
Target: 5'- -----gGGGGCAGAGgGCGuGCAUg- -3' miRNA: 3'- uaauaaUCCCGUUUCaCGCuCGUGac -5' |
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5320 | 5' | -48.6 | NC_001798.1 | + | 150087 | 0.77 | 0.705464 |
Target: 5'- -----gGGGGCGAGGgGCGAGCGCg- -3' miRNA: 3'- uaauaaUCCCGUUUCaCGCUCGUGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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