Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 5324 | 3' | -57 | NC_001798.1 | + | 144432 | 0.73 | 0.523236 |
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Target: 5'- gGGGGGGUuggGGGGGGAacGCUGACcgGCgugCGu -3' miRNA: 3'- gCCCCUCA---UCCCCCU--CGAUUG--UGa--GC- -5' |
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| 5324 | 3' | -57 | NC_001798.1 | + | 7133 | 0.74 | 0.430349 |
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Target: 5'- gCGGGGAG-AGGGGGGGggGGCGggCGg -3' miRNA: 3'- -GCCCCUCaUCCCCCUCgaUUGUgaGC- -5' |
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| 5324 | 3' | -57 | NC_001798.1 | + | 68921 | 0.75 | 0.387668 |
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Target: 5'- gGGGGGGgcGGGGGcgGGCgGGgGCUCGg -3' miRNA: 3'- gCCCCUCauCCCCC--UCGaUUgUGAGC- -5' |
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| 5324 | 3' | -57 | NC_001798.1 | + | 43102 | 0.77 | 0.325424 |
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Target: 5'- uCGGGGGcgcucGgcGGGGGGGCggaagaGACGCUCGg -3' miRNA: 3'- -GCCCCU-----CauCCCCCUCGa-----UUGUGAGC- -5' |
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| 5324 | 3' | -57 | NC_001798.1 | + | 139559 | 0.82 | 0.162567 |
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Target: 5'- uCGGGGGGUGGGGGG-GCUGGCGagcCGg -3' miRNA: 3'- -GCCCCUCAUCCCCCuCGAUUGUga-GC- -5' |
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| 5324 | 3' | -57 | NC_001798.1 | + | 121924 | 1.1 | 0.002122 |
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Target: 5'- aCGGGGAGUAGGGGGAGCUAACACUCGg -3' miRNA: 3'- -GCCCCUCAUCCCCCUCGAUUGUGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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