Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5324 | 3' | -57 | NC_001798.1 | + | 7133 | 0.74 | 0.430349 |
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Target: 5'- gCGGGGAG-AGGGGGGGggGGCGggCGg -3' miRNA: 3'- -GCCCCUCaUCCCCCUCgaUUGUgaGC- -5' |
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| 5324 | 3' | -57 | NC_001798.1 | + | 5902 | 0.68 | 0.779874 |
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Target: 5'- uCGGGG-GUGGGGGcGGCUu---CUCGu -3' miRNA: 3'- -GCCCCuCAUCCCCcUCGAuuguGAGC- -5' |
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| 5324 | 3' | -57 | NC_001798.1 | + | 4662 | 0.67 | 0.832187 |
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Target: 5'- uGGGGGGcgAGGGGcGAGCgcggGGCGg-CGg -3' miRNA: 3'- gCCCCUCa-UCCCC-CUCGa---UUGUgaGC- -5' |
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| 5324 | 3' | -57 | NC_001798.1 | + | 2573 | 0.72 | 0.562558 |
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Target: 5'- cCGGGGAaaAGGGGGGGcCUGAgACcCGg -3' miRNA: 3'- -GCCCCUcaUCCCCCUC-GAUUgUGaGC- -5' |
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| 5324 | 3' | -57 | NC_001798.1 | + | 985 | 0.68 | 0.761314 |
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Target: 5'- uGGGGcGGaAGGGGGcGCUGcggcccGCGCUCc -3' miRNA: 3'- gCCCC-UCaUCCCCCuCGAU------UGUGAGc -5' |
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| 5324 | 3' | -57 | NC_001798.1 | + | 370 | 0.72 | 0.532976 |
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Target: 5'- gGGGGGGUguuuuuugGGGGGGGGCgcgAAgGCgggCGg -3' miRNA: 3'- gCCCCUCA--------UCCCCCUCGa--UUgUGa--GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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