Results 101 - 109 of 109 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5327 | 5' | -55.1 | NC_001798.1 | + | 2663 | 0.7 | 0.757497 |
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Target: 5'- gCGCGccuccCGCGGCGCGgag-GCGGGcGCGg -3' miRNA: 3'- -GUGCu----GUGCUGCGCaaaaCGCCC-CGU- -5' |
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| 5327 | 5' | -55.1 | NC_001798.1 | + | 2628 | 0.66 | 0.941728 |
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Target: 5'- gGCGGCGCccuGGC-CGgg--GCGGGGCu -3' miRNA: 3'- gUGCUGUG---CUGcGCaaaaCGCCCCGu -5' |
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| 5327 | 5' | -55.1 | NC_001798.1 | + | 2544 | 0.66 | 0.920805 |
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Target: 5'- gGgGGCGCGGCccccgcgggagggGCGgcc-GCGGGGCGg -3' miRNA: 3'- gUgCUGUGCUG-------------CGCaaaaCGCCCCGU- -5' |
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| 5327 | 5' | -55.1 | NC_001798.1 | + | 2514 | 0.75 | 0.50643 |
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Target: 5'- gGCGGCcgcgGCGGCgGCGUcg-GCGGGGCGg -3' miRNA: 3'- gUGCUG----UGCUG-CGCAaaaCGCCCCGU- -5' |
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| 5327 | 5' | -55.1 | NC_001798.1 | + | 2249 | 0.71 | 0.694379 |
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Target: 5'- gGCGGCG-GGCGCGccgccgggggGCGGGGCGg -3' miRNA: 3'- gUGCUGUgCUGCGCaaaa------CGCCCCGU- -5' |
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| 5327 | 5' | -55.1 | NC_001798.1 | + | 1627 | 0.68 | 0.883695 |
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Target: 5'- cCGCGGCagaggcgcagcgGCGGCGCGU----CGGGGUAc -3' miRNA: 3'- -GUGCUG------------UGCUGCGCAaaacGCCCCGU- -5' |
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| 5327 | 5' | -55.1 | NC_001798.1 | + | 1541 | 0.71 | 0.728329 |
|
Target: 5'- gCACGGCGCGGCG-GUacucgcGCGGGGaCAu -3' miRNA: 3'- -GUGCUGUGCUGCgCAaaa---CGCCCC-GU- -5' |
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| 5327 | 5' | -55.1 | NC_001798.1 | + | 1226 | 0.67 | 0.903582 |
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Target: 5'- cCGCGGC-CGACgcccaGCGUaucUGCGgGGGCGg -3' miRNA: 3'- -GUGCUGuGCUG-----CGCAaa-ACGC-CCCGU- -5' |
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| 5327 | 5' | -55.1 | NC_001798.1 | + | 58 | 0.66 | 0.946224 |
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Target: 5'- gGgGGCGCGAagGCGggcgGCGGcGGCGg -3' miRNA: 3'- gUgCUGUGCUg-CGCaaaaCGCC-CCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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