Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5335 | 3' | -49.8 | NC_001798.1 | + | 128512 | 0.66 | 0.998715 |
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Target: 5'- gCCCa---GUCGUcgGCGGUGAUGUucGGGc -3' miRNA: 3'- -GGGcaucUAGCAa-CGCCACUACA--UCU- -5' |
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| 5335 | 3' | -49.8 | NC_001798.1 | + | 39125 | 0.66 | 0.998139 |
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Target: 5'- gCCCGcGGGUUGgcUGCGaauGUGcgGUAGAg -3' miRNA: 3'- -GGGCaUCUAGCa-ACGC---CACuaCAUCU- -5' |
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| 5335 | 3' | -49.8 | NC_001798.1 | + | 65810 | 0.67 | 0.996876 |
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Target: 5'- cCCCGUugcgggcgaGGAUggcgcucaCGUUGcCGGUGAUGgcccGGAg -3' miRNA: 3'- -GGGCA---------UCUA--------GCAAC-GCCACUACa---UCU- -5' |
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| 5335 | 3' | -49.8 | NC_001798.1 | + | 57930 | 0.67 | 0.996324 |
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Target: 5'- cCCCGgGGAUCGgaGCGGUag-GgcGAa -3' miRNA: 3'- -GGGCaUCUAGCaaCGCCAcuaCauCU- -5' |
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| 5335 | 3' | -49.8 | NC_001798.1 | + | 56172 | 0.68 | 0.99109 |
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Target: 5'- gCCCGggGGAcggCGggGgGGUGggGUGGGg -3' miRNA: 3'- -GGGCa-UCUa--GCaaCgCCACuaCAUCU- -5' |
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| 5335 | 3' | -49.8 | NC_001798.1 | + | 89546 | 0.69 | 0.97899 |
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Target: 5'- gUCGUAGAUgG-UGCGgGUGAUGUuGAu -3' miRNA: 3'- gGGCAUCUAgCaACGC-CACUACAuCU- -5' |
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| 5335 | 3' | -49.8 | NC_001798.1 | + | 113954 | 0.72 | 0.928557 |
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Target: 5'- gCCCGUGGGUUGUUagucgcguccgccgGCGGUGAggcguUGUgcgGGAg -3' miRNA: 3'- -GGGCAUCUAGCAA--------------CGCCACU-----ACA---UCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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