Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5337 | 5' | -50.7 | NC_001798.1 | + | 78937 | 0.66 | 0.993021 |
Target: 5'- aGUGGGAcGAGGUcugggGUCGCUuuggccgcguCCGGggGg -3' miRNA: 3'- gCAUCCUaUUCCA-----UAGCGG----------GGUCuuU- -5' |
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5337 | 5' | -50.7 | NC_001798.1 | + | 102061 | 0.66 | 0.991952 |
Target: 5'- gCGUGGGGgugguGGUAggGCCCCAc--- -3' miRNA: 3'- -GCAUCCUauu--CCAUagCGGGGUcuuu -5' |
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5337 | 5' | -50.7 | NC_001798.1 | + | 46903 | 0.68 | 0.97279 |
Target: 5'- cCGUuGGAcAAGG-AUCGCCCUGGGc- -3' miRNA: 3'- -GCAuCCUaUUCCaUAGCGGGGUCUuu -5' |
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5337 | 5' | -50.7 | NC_001798.1 | + | 79200 | 0.7 | 0.926934 |
Target: 5'- --cGGGAUGAGGUGgugcgcagggUGCCCUGGGAGa -3' miRNA: 3'- gcaUCCUAUUCCAUa---------GCGGGGUCUUU- -5' |
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5337 | 5' | -50.7 | NC_001798.1 | + | 101945 | 0.74 | 0.777908 |
Target: 5'- aCG-AGGGUGGGGggacCGCCCCGGAu- -3' miRNA: 3'- -GCaUCCUAUUCCaua-GCGGGGUCUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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