Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5368 | 5' | -51.6 | NC_001798.1 | + | 53830 | 0.66 | 0.990527 |
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Target: 5'- aCGUGUGUCgccgccugggcCCCGccgaucgccGGUUCGugGCGCu -3' miRNA: 3'- -GCACGCAGaa---------GGGU---------CUAAGCugUGUG- -5' |
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| 5368 | 5' | -51.6 | NC_001798.1 | + | 45298 | 0.66 | 0.988037 |
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Target: 5'- aCGUGCucGUC-UCCCAGGccaUUCGGaGCAUg -3' miRNA: 3'- -GCACG--CAGaAGGGUCU---AAGCUgUGUG- -5' |
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| 5368 | 5' | -51.6 | NC_001798.1 | + | 16334 | 0.66 | 0.988037 |
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Target: 5'- --cGCGg--UCCCAGAU-CGGCACcaGCa -3' miRNA: 3'- gcaCGCagaAGGGUCUAaGCUGUG--UG- -5' |
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| 5368 | 5' | -51.6 | NC_001798.1 | + | 119641 | 0.67 | 0.984707 |
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Target: 5'- uGUGUGUCgaaUCUgGGAa--GACGCACg -3' miRNA: 3'- gCACGCAGa--AGGgUCUaagCUGUGUG- -5' |
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| 5368 | 5' | -51.6 | NC_001798.1 | + | 137770 | 0.67 | 0.980714 |
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Target: 5'- uCGUGC----UCCCAGAggcgCGACAgGCg -3' miRNA: 3'- -GCACGcagaAGGGUCUaa--GCUGUgUG- -5' |
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| 5368 | 5' | -51.6 | NC_001798.1 | + | 6872 | 0.67 | 0.980714 |
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Target: 5'- aCGUGCGaUCggaUCCCGcGAgUCGAaauccCACACa -3' miRNA: 3'- -GCACGC-AGa--AGGGU-CUaAGCU-----GUGUG- -5' |
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| 5368 | 5' | -51.6 | NC_001798.1 | + | 87987 | 0.68 | 0.97047 |
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Target: 5'- --cGCGcC-UCCCGGGgggUCGGCAUGCg -3' miRNA: 3'- gcaCGCaGaAGGGUCUa--AGCUGUGUG- -5' |
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| 5368 | 5' | -51.6 | NC_001798.1 | + | 127521 | 0.69 | 0.948589 |
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Target: 5'- cCG-GCGccaccuUCgcaaUCCCaAGAUUCGGCGCGCg -3' miRNA: 3'- -GCaCGC------AGa---AGGG-UCUAAGCUGUGUG- -5' |
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| 5368 | 5' | -51.6 | NC_001798.1 | + | 73580 | 0.71 | 0.878412 |
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Target: 5'- -aUGUGUCUUCCCGGGaaaCcACACACa -3' miRNA: 3'- gcACGCAGAAGGGUCUaa-GcUGUGUG- -5' |
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| 5368 | 5' | -51.6 | NC_001798.1 | + | 138492 | 0.77 | 0.59136 |
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Target: 5'- --cGCGUgUUCCCGGAgggCGugGCACg -3' miRNA: 3'- gcaCGCAgAAGGGUCUaa-GCugUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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