Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 5393 | 5' | -56.6 | NC_001798.1 | + | 12566 | 0.74 | 0.48515 |
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Target: 5'- gGCGCGu-CGUCAUCCCggaUGUGGGGGAGa -3' miRNA: 3'- -CGUGUuuGUGGUGGGG---ACACCCCCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 67159 | 0.74 | 0.448053 |
|
Target: 5'- gGCGC-AGCACUcgugGCCCCaUGgaaagGGGGGAGg -3' miRNA: 3'- -CGUGuUUGUGG----UGGGG-ACa----CCCCCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 112923 | 0.75 | 0.430103 |
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Target: 5'- cCGCGGAUACUACCCggGUGGGGGu- -3' miRNA: 3'- cGUGUUUGUGGUGGGgaCACCCCCuc -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 105980 | 0.76 | 0.378899 |
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Target: 5'- cCGCAgcGACGCCuacgACCCCUGgggaGGGGGGGu -3' miRNA: 3'- cGUGU--UUGUGG----UGGGGACa---CCCCCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 29570 | 0.79 | 0.239519 |
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Target: 5'- --gUAAAUGCCGCCCCUuUGGGGGAGu -3' miRNA: 3'- cguGUUUGUGGUGGGGAcACCCCCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 16199 | 0.83 | 0.149412 |
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Target: 5'- cGCACGGgccgcccuccGCACgCGCCgCCUGUGGGGGGGc -3' miRNA: 3'- -CGUGUU----------UGUG-GUGG-GGACACCCCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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