Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 5393 | 5' | -56.6 | NC_001798.1 | + | 5614 | 0.66 | 0.880324 |
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Target: 5'- cCGCGGGCGgcuCCGCCCCaaagGGGGcGGGg -3' miRNA: 3'- cGUGUUUGU---GGUGGGGaca-CCCC-CUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 138918 | 0.66 | 0.880324 |
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Target: 5'- gGCGCAAAgcagucUACCACCCC-GUGGGc--- -3' miRNA: 3'- -CGUGUUU------GUGGUGGGGaCACCCccuc -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 85430 | 0.66 | 0.872458 |
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Target: 5'- uGUACAAGCAggccuaccgccucUCGCCCCaGUGGGuGGc- -3' miRNA: 3'- -CGUGUUUGU-------------GGUGGGGaCACCC-CCuc -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 65672 | 0.66 | 0.86583 |
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Target: 5'- gGUACAcGCA-CGCCCCgGUGGGGc-- -3' miRNA: 3'- -CGUGUuUGUgGUGGGGaCACCCCcuc -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 119732 | 0.66 | 0.863582 |
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Target: 5'- cGCACcccaACCACCCaaccaacggacaaaCUGUuguaGGGGGGGa -3' miRNA: 3'- -CGUGuuugUGGUGGG--------------GACA----CCCCCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 74697 | 0.66 | 0.900418 |
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Target: 5'- gGCACccaaACCGCCCCcccgaugaugUGUGGGcagcagcccuGGAGg -3' miRNA: 3'- -CGUGuuugUGGUGGGG----------ACACCC----------CCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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