Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5393 | 5' | -56.6 | NC_001798.1 | + | 137130 | 0.68 | 0.791067 |
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Target: 5'- -aACGAACcgcaACCGCCCCc-UGGGGGuGa -3' miRNA: 3'- cgUGUUUG----UGGUGGGGacACCCCCuC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 138918 | 0.66 | 0.880324 |
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Target: 5'- gGCGCAAAgcagucUACCACCCC-GUGGGc--- -3' miRNA: 3'- -CGUGUUU------GUGGUGGGGaCACCCccuc -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 150053 | 0.69 | 0.744207 |
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Target: 5'- gGCGC-GGCGCCGCCCUcuUGgcccccacccccUGGGGGGc -3' miRNA: 3'- -CGUGuUUGUGGUGGGG--AC------------ACCCCCUc -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 150173 | 0.71 | 0.634149 |
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Target: 5'- -gGCGGGcCGCCGCCCCcuccgcggcGUGGGGGGc -3' miRNA: 3'- cgUGUUU-GUGGUGGGGa--------CACCCCCUc -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 153047 | 0.73 | 0.522673 |
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Target: 5'- cGCGCGguuggccGGCGCCGcCCCCUGgggcGGGcGGAGc -3' miRNA: 3'- -CGUGU-------UUGUGGU-GGGGACa---CCC-CCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 154488 | 0.66 | 0.886564 |
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Target: 5'- cCGCGGGCGCCGCCCCUccccccgcgcgccGcGGGGc-- -3' miRNA: 3'- cGUGUUUGUGGUGGGGA-------------CaCCCCcuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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