Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5393 | 5' | -56.6 | NC_001798.1 | + | 5023 | 0.68 | 0.800049 |
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Target: 5'- cCGCcGACAUCGCCUCcgaccggggUGUGcGGGGGGg -3' miRNA: 3'- cGUGuUUGUGGUGGGG---------ACAC-CCCCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 3876 | 0.69 | 0.72472 |
|
Target: 5'- cGUAC-AGCACgCGCCCCgggGGcGGGGGg -3' miRNA: 3'- -CGUGuUUGUG-GUGGGGacaCC-CCCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 2611 | 0.68 | 0.808881 |
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Target: 5'- gGCGCgGGGCGCCGCccggcggcgCCCUGgccGGGGcGGGg -3' miRNA: 3'- -CGUG-UUUGUGGUG---------GGGACa--CCCC-CUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 2077 | 0.69 | 0.72472 |
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Target: 5'- cGCGCucGGCggACCACUCCg--GGGGGGGc -3' miRNA: 3'- -CGUGu-UUG--UGGUGGGGacaCCCCCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 568 | 0.66 | 0.887246 |
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Target: 5'- cGCGCcccguGCcuucccucCCGCUCCUGcGGGGGGGc -3' miRNA: 3'- -CGUGuu---UGu-------GGUGGGGACaCCCCCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 150 | 0.66 | 0.886564 |
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Target: 5'- cCGCGGGCGCCGCCCCUccccccgcgcgccGcGGGGc-- -3' miRNA: 3'- cGUGUUUGUGGUGGGGA-------------CaCCCCcuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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