Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5393 | 5' | -56.6 | NC_001798.1 | + | 36395 | 0.67 | 0.834392 |
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Target: 5'- uGCGC-GGCGCCGgggccCCCCUGccGGGcGGGGc -3' miRNA: 3'- -CGUGuUUGUGGU-----GGGGACa-CCC-CCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 26269 | 0.67 | 0.841735 |
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Target: 5'- cGCACucGCACCGCgCCUGcgcgcgcUGGGGccuGGGc -3' miRNA: 3'- -CGUGuuUGUGGUGgGGAC-------ACCCC---CUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 68585 | 0.67 | 0.849715 |
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Target: 5'- cGCuCGAGgGCCGCCuCCaGUccgcgcgGGGGGAGc -3' miRNA: 3'- -CGuGUUUgUGGUGG-GGaCA-------CCCCCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 69174 | 0.67 | 0.850502 |
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Target: 5'- aGCcCGAugGCgGgCCagGUGGGGGAGg -3' miRNA: 3'- -CGuGUUugUGgUgGGgaCACCCCCUC- -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 6675 | 0.67 | 0.850502 |
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Target: 5'- gGC-CGGGCGCCGCCUUcGUGGacGGGAc -3' miRNA: 3'- -CGuGUUUGUGGUGGGGaCACC--CCCUc -5' |
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| 5393 | 5' | -56.6 | NC_001798.1 | + | 74697 | 0.66 | 0.900418 |
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Target: 5'- gGCACccaaACCGCCCCcccgaugaugUGUGGGcagcagcccuGGAGg -3' miRNA: 3'- -CGUGuuugUGGUGGGG----------ACACCC----------CCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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